TimeLAPSE 2.66
LINK >>> https://geags.com/2tki7t
For quantitative phase imaging (QPI) based on transport-of-intensity equation (TIE), partially coherent illumination provides speckle-free imaging, compatibility with brightfield microscopy, and transverse resolution beyond coherent diffraction limit. Unfortunately, in a conventional microscope with circular illumination aperture, partial coherence tends to diminish the phase contrast, exacerbating the inherent noise-to-resolution tradeoff in TIE imaging, resulting in strong low-frequency artifacts and compromised imaging resolution. Here, we demonstrate how these issues can be effectively addressed by replacing the conventional circular illumination aperture with an annular one. The matched annular illumination not only strongly boosts the phase contrast for low spatial frequencies, but significantly improves the practical imaging resolution to near the incoherent diffraction limit. By incorporating high-numerical aperture (NA) illumination as well as high-NA objective, it is shown, for the first time, that TIE phase imaging can achieve a transverse resolution up to 208 nm, corresponding to an effective NA of 2.66. Time-lapse imaging of in vitro Hela cells revealing cellular morphology and subcellular dynamics during cells mitosis and apoptosis is exemplified. Given its capability for high-resolution QPI as well as the compatibility with widely available brightfield microscopy hardware, the proposed approach is expected to be adopted by the wider biology and medicine community.
You should really try out After Effects for editing your timelapses. The 64-bit engine now is so powerful that you can drop in unedited RAW files as a sequence, apply changes to the CR2 files and then add some MBL afterwards.
Meanwhile, levels of carbon dioxide also continue to increase at historically high rates. The global surface average for carbon dioxide during 2021 was 414.7 parts per million (ppm), which is an increase of 2.66 ppm over the 2020 average. This marks the 10th consecutive year that carbon dioxide increased by more than 2 parts per million, which represents the fastest sustained rate of increase in the 63 years since monitoring began. 59ce067264
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